Title

Field Surveys for the Detection of Chytridiomycosis in North Georgia Amphibian Populations

Proposal Type

Poster

Additional Presenter Information

N.L. Hyslop, Ph.D.

Associate Professor, Biology

University of North Georgia

Gainesville, GA 30566

678-717-3538

nhyslop@ung.edu

Jeanelle M. Morgan, Ph.D.

Associate Professor and Associate Department Head of Biology University of North Georgia – Gainesville Campus

161 Science Building

678.717.3706

Keywords

Disease, amphibians, declines, Georgia, population

Subject Area

Biology

Start Date

11-11-2016 11:45 AM

End Date

11-11-2016 1:15 PM

Description/Abstract

Chytridiomycosis is a fungal disease caused by the pathogen Batrachochytrium dendrobatidis (Bd), which is a contributing factor to global amphibian population declines. Although Bd is distributed globally, little research has been conducted on the occurrence of Bd in north Georgia’s amphibian populations. We surveyed for the presence of Bd in amphibian populations in the northeast Georgia Piedmont region at 3 different locations using active night searches and passive sampling techniques from spring 2013 through Spring 2016. During night searches, amphibians were located in wetlands and captured by hand. We used poly-vinyl chloride (PVC) pipe traps for passive sampling during the day. Following captures, we collected environmental and physical data from each individual, swabbed the skin for Bd detection using sterile polyester tipped swabs, and released individuals at their capture site. Collected skin swabs were analyzed using polymerase chain reaction (PCR) testing to determine the presence of Bd. To date, PCR techniques have detected a positive Bd sample in a Green Treefrog (Hyla cinerea) at one of the sites. Sampling, along with a capture-mark-recapture investigation of Green Treefrogs using PVC passive sampling will continue throughout 2016 to contribute to conservation efforts and knowledge of Bd in the region.

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Nov 11th, 11:45 AM Nov 11th, 1:15 PM

Field Surveys for the Detection of Chytridiomycosis in North Georgia Amphibian Populations

Chytridiomycosis is a fungal disease caused by the pathogen Batrachochytrium dendrobatidis (Bd), which is a contributing factor to global amphibian population declines. Although Bd is distributed globally, little research has been conducted on the occurrence of Bd in north Georgia’s amphibian populations. We surveyed for the presence of Bd in amphibian populations in the northeast Georgia Piedmont region at 3 different locations using active night searches and passive sampling techniques from spring 2013 through Spring 2016. During night searches, amphibians were located in wetlands and captured by hand. We used poly-vinyl chloride (PVC) pipe traps for passive sampling during the day. Following captures, we collected environmental and physical data from each individual, swabbed the skin for Bd detection using sterile polyester tipped swabs, and released individuals at their capture site. Collected skin swabs were analyzed using polymerase chain reaction (PCR) testing to determine the presence of Bd. To date, PCR techniques have detected a positive Bd sample in a Green Treefrog (Hyla cinerea) at one of the sites. Sampling, along with a capture-mark-recapture investigation of Green Treefrogs using PVC passive sampling will continue throughout 2016 to contribute to conservation efforts and knowledge of Bd in the region.