Title

Genetic Engineering of Model Reflectin-based Protein Materials

Faculty Mentor(s)

Holly E. Carpenter Desai

Location

Library Technology Center David L. Potter Special Collections Room 382

Start Date

27-3-2012 12:30 PM

End Date

27-3-2012 1:45 PM

Description/Abstract

In an attempt toward improving our understanding of a functional unit that drives self-assembly of reflectin-based protein materials, a genetic engineering approach was applied for synthesis of a reflectin-based sequence of interest. A dimer, based on the reflectin 1a, domain 3 sequence, was constructed and cloned into an expression vector. Protein expression of the domain 3 (D3) dimer protein was carried out in a bacterial host and protein expression levels were evaluated with western blotting. Characterization of the D3 dimer protein is currently underway. Faculty Adviser: Holly E. Carpenter Desai

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Mar 27th, 12:30 PM Mar 27th, 1:45 PM

Genetic Engineering of Model Reflectin-based Protein Materials

Library Technology Center David L. Potter Special Collections Room 382

In an attempt toward improving our understanding of a functional unit that drives self-assembly of reflectin-based protein materials, a genetic engineering approach was applied for synthesis of a reflectin-based sequence of interest. A dimer, based on the reflectin 1a, domain 3 sequence, was constructed and cloned into an expression vector. Protein expression of the domain 3 (D3) dimer protein was carried out in a bacterial host and protein expression levels were evaluated with western blotting. Characterization of the D3 dimer protein is currently underway. Faculty Adviser: Holly E. Carpenter Desai