Event Title

Identification and characterization of three novel strains of bacteria that can be used for bioremediation of cyclic alkane, cyclic aromatic hydrocarbons and polyhydroxyalkanoates.

Faculty Mentor

Mautusi Mitra

Proposal Type

Oral Presentation

Start Date

2-11-2019 8:00 AM

End Date

2-11-2019 9:00 AM

Location

Nesbitt 3201

Abstract

Identification and characterization of three novel strains of bacteria that can be used for bioremediation of cyclic alkane, cyclic aromatic hydrocarbons and polyhydroxyalkanoates.

Taylor Box, Kevin Nguyen, Seth Hamby, Taylor Berry, Sovi Wellons, Erin Duckett and Mautusi Mitra

Three different bacterial strains were isolated from the contaminated TAP (Tris Acetate Phosphate) media plates of the green micro-alga Chlamydomonas reinhardtii in our research lab. These bacterial strains were named based on the Chlamydomonas strains they contaminated: CC4533, Clip 185, and LMJSG0182. These bacterial were able to grow on the algal media plates as these strains could utilize acetate as an alternative carbon source like Chlamydomonas. Several microbiological tests were conducted to characterize the bacteria biochemically. We amplified and sequenced partially the 16S rRNA gene sequences of these three bacterial strains. The sequencing data showed that these three bacteria are novel strains of bacterial species: Sphingobium sp. strain MK41, Microbacterium binotii, and an uncultured bacterium clone LIB091_C05_1243 that is closely related to the genus Acidovorax sp. We tested the abilities of these three bacterial strains to utilize cyclic alkane, poly-aromatic hydrocarbons, mono-aromatic hydrocarbons and poly-hydroxyalkanoates as the sole carbon source on TP (Tris Phosphate) + 0.1% ammonium chloride media plates. The results show that both CC4533 and LMJSG0182 strains have the potential for bioremediation, with CC4533 being the most promising candidate. We will be presenting our biochemical and molecular research at the GURC meeting.

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Nov 2nd, 8:00 AM Nov 2nd, 9:00 AM

Identification and characterization of three novel strains of bacteria that can be used for bioremediation of cyclic alkane, cyclic aromatic hydrocarbons and polyhydroxyalkanoates.

Nesbitt 3201

Identification and characterization of three novel strains of bacteria that can be used for bioremediation of cyclic alkane, cyclic aromatic hydrocarbons and polyhydroxyalkanoates.

Taylor Box, Kevin Nguyen, Seth Hamby, Taylor Berry, Sovi Wellons, Erin Duckett and Mautusi Mitra

Three different bacterial strains were isolated from the contaminated TAP (Tris Acetate Phosphate) media plates of the green micro-alga Chlamydomonas reinhardtii in our research lab. These bacterial strains were named based on the Chlamydomonas strains they contaminated: CC4533, Clip 185, and LMJSG0182. These bacterial were able to grow on the algal media plates as these strains could utilize acetate as an alternative carbon source like Chlamydomonas. Several microbiological tests were conducted to characterize the bacteria biochemically. We amplified and sequenced partially the 16S rRNA gene sequences of these three bacterial strains. The sequencing data showed that these three bacteria are novel strains of bacterial species: Sphingobium sp. strain MK41, Microbacterium binotii, and an uncultured bacterium clone LIB091_C05_1243 that is closely related to the genus Acidovorax sp. We tested the abilities of these three bacterial strains to utilize cyclic alkane, poly-aromatic hydrocarbons, mono-aromatic hydrocarbons and poly-hydroxyalkanoates as the sole carbon source on TP (Tris Phosphate) + 0.1% ammonium chloride media plates. The results show that both CC4533 and LMJSG0182 strains have the potential for bioremediation, with CC4533 being the most promising candidate. We will be presenting our biochemical and molecular research at the GURC meeting.