Title
Genetic Engineering of Model Reflectin-based Protein Materials
Faculty Mentor(s)
Holly E. Carpenter Desai
Location
Library Technology Center David L. Potter Special Collections Room 382
Start Date
27-3-2012 12:30 PM
End Date
27-3-2012 1:45 PM
Description/Abstract
In an attempt toward improving our understanding of a functional unit that drives self-assembly of reflectin-based protein materials, a genetic engineering approach was applied for synthesis of a reflectin-based sequence of interest. A dimer, based on the reflectin 1a, domain 3 sequence, was constructed and cloned into an expression vector. Protein expression of the domain 3 (D3) dimer protein was carried out in a bacterial host and protein expression levels were evaluated with western blotting. Characterization of the D3 dimer protein is currently underway. Faculty Adviser: Holly E. Carpenter Desai
Genetic Engineering of Model Reflectin-based Protein Materials
Library Technology Center David L. Potter Special Collections Room 382
In an attempt toward improving our understanding of a functional unit that drives self-assembly of reflectin-based protein materials, a genetic engineering approach was applied for synthesis of a reflectin-based sequence of interest. A dimer, based on the reflectin 1a, domain 3 sequence, was constructed and cloned into an expression vector. Protein expression of the domain 3 (D3) dimer protein was carried out in a bacterial host and protein expression levels were evaluated with western blotting. Characterization of the D3 dimer protein is currently underway. Faculty Adviser: Holly E. Carpenter Desai