Panel C: Immunolocalization of Polypeptide Growth Factors in Estradiol-17β- Mediated Oviductal Proliferation and Differentiation in the Mediterranean Gecko, Hemidactylus turcicus

Jessica Nix
Josie Ayers, University of North Georgia
David Dennard, University of North Georgia
Emily Harris, University of North Georgia
Stuart Hardman, University of North Georgia
Emily Muñoz, University of North Georgia
Cathy Whiting, University of North Georgia

Description/Abstract

Estrogens are essential for normal growth and differentiation of the vertebrate oviduct. The observation that estradiol-17β (E2) initiated little or no response in isolated mammalian uterine cells has led to the hypothesis that paracrine growth factors mediate E2-induced proliferation and differentiation of uterine cells. Polypeptide growth factors such as insulin-like growth-factor I (IGF-1), and epidermal growth factor (EGF) have been implicated in this process. In a preliminary morphometric study in reptiles, Mediterranean geckos, Hemidactylus turcicus, were ovariectomized and implanted with E2, IGF-1, or control-pellets. After a fourteen-day treatment period, the oviductal tissues were processed histologically. Morphometrical data analysis demonstrated a significantly higher endometrial gland circumference in both E2- and IGF-1-treated animals compared to control animals. These data support the hypothesis that the E2-induced changes observed in the vertebrate oviduct are mediated by growth factors. The purpose of this study was to use immunohistochemistry to localize IGF-1 and EGF in the oviduct of ovariectomized geckos treated with E2. Using prepared slides from this preliminary study, we stained the oviductal tissues using polyclonal antibodies (anti-IGF-1 and anti-EGF) and a VECTASTAIN® Universal Quick-Kit, an avidin/biotin based horseradish-peroxidase amplification-system, to localize the growth factors. Preliminary analysis of the slides demonstrated the presence of IGF-1 throughout the endometrium and myometrium. The presence of EGF was inconclusive and requires further analysis. Our novel experiment provides further evidence that E2-induced cyclic oviductal changes in reptiles are mediated, in part, by IGF-1. Further research is needed to examine the role of additional growth factors in this process.

 
Mar 25th, 10:00 AM Mar 25th, 11:00 AM

Panel C: Immunolocalization of Polypeptide Growth Factors in Estradiol-17β- Mediated Oviductal Proliferation and Differentiation in the Mediterranean Gecko, Hemidactylus turcicus

Nesbitt 3202

Estrogens are essential for normal growth and differentiation of the vertebrate oviduct. The observation that estradiol-17β (E2) initiated little or no response in isolated mammalian uterine cells has led to the hypothesis that paracrine growth factors mediate E2-induced proliferation and differentiation of uterine cells. Polypeptide growth factors such as insulin-like growth-factor I (IGF-1), and epidermal growth factor (EGF) have been implicated in this process. In a preliminary morphometric study in reptiles, Mediterranean geckos, Hemidactylus turcicus, were ovariectomized and implanted with E2, IGF-1, or control-pellets. After a fourteen-day treatment period, the oviductal tissues were processed histologically. Morphometrical data analysis demonstrated a significantly higher endometrial gland circumference in both E2- and IGF-1-treated animals compared to control animals. These data support the hypothesis that the E2-induced changes observed in the vertebrate oviduct are mediated by growth factors. The purpose of this study was to use immunohistochemistry to localize IGF-1 and EGF in the oviduct of ovariectomized geckos treated with E2. Using prepared slides from this preliminary study, we stained the oviductal tissues using polyclonal antibodies (anti-IGF-1 and anti-EGF) and a VECTASTAIN® Universal Quick-Kit, an avidin/biotin based horseradish-peroxidase amplification-system, to localize the growth factors. Preliminary analysis of the slides demonstrated the presence of IGF-1 throughout the endometrium and myometrium. The presence of EGF was inconclusive and requires further analysis. Our novel experiment provides further evidence that E2-induced cyclic oviductal changes in reptiles are mediated, in part, by IGF-1. Further research is needed to examine the role of additional growth factors in this process.